Characteristics of ammonium and nitrate fluxes along the roots of Picea asperata

Nitrogen (N), ammonium (NH4+) and nitrate (NO3-), is one of the key determinants for plant growth. The interaction of both ions displays a significant effect on their uptake in some species. In the current study, net fluxes of NH4+ and NO3- along the roots of Picea asperata were determined using a Non-invasive Micro-test Technology (NMT). Besides, we examined the interaction of NH4+ and NO3- on the fluxes of both ions, and the plasma membrane (PM) H+-ATPases and nitrate reductase (NR) were taken into account as well. The results demonstrated that the maximal net NH4+ and NO3- influxes were detected at 13-15 mm and 8-10.5 mm from the root apex, respectively. Net NH4+ influx was significantly stimulated with the presence of NO3-, whereas NH4+ exhibited a markedly negative effect on NO3- uptake in the roots of P. asperata. Also, our results indicated that PM H+-ATPases and NR play a key role in the control of N uptake