Assessment of DNA separation and recovery using DNA profiles from a temperature controlled differential extraction

In 2010, Bright et al. created two person mixtures to determine how effective traditional differential extraction was in determining mixtures by examining mixture proportion variation by using the peak heights from each sample. This project aims to follow that method, however, in this case using a Temperature Controlled Differential Extraction (TCDE) to analyze post coital swabs in place of a traditional differential extraction. The project also aims to determine how efficient the separation of sperm cells from epithelial cells was by comparing the mixture proportion mean of male deoxyribonucleic acid (DNA) from an Acrosolv digest that did not undergo the TCDE to the proportion of male DNA from the TCDE. The amount of DNA remaining on a swab after undergoing the TCDE was also assessed as a material fraction. Many of the material fractions generated a mixture in their profiles and thus enough DNA to generate a male profile was remaining on the swab after the TCDE in almost all cases. The sperm fractions were mostly single source male profiles or profiles with the male DNA as a major contributor and the female DNA as a minor contributor