In native tissues, relatively low protein expression levels and overlapping sensitivities of antibodies within families of GPCRs present challenges to transfer of knowledge of receptor mechanisms from cell culture. In contrast, in recombinant systems epitope tags and fluorescent proteins provide powerful and sensitive means of tracking the concentration, location and movement of receptors. Two approaches have now started to close this gap. First is the use of fluorescent ligands, which can be tested and validated in recombinant systems [1] and then employed in intact, live native tissue using confocal microscopy [2-4]. Agonist and antagonist ligands can be employed, each conferring advantages and disadvantages. The second approach is the cr...
This review is based on the JR Vane Medal Lecture presented at the BPS Winter Meeting in December 20...
A better understanding of the mechanisms governing receptor trafficking between the plasma membrane ...
There is a need to develop new techniques for quantitative measurement of receptors expression on pa...
This chapter reviews and compares the strengths, limitations, and potential for development of the t...
1: This study examines the cellular localization of α1-adrenoceptors and demonstrates that binding t...
The discovery of beta-adrenoceptors in previously unsuspected cell types is contributing to the reth...
Background and purpose: Pharmacological analysis of synergism or functional antagonism between diffe...
A fluorescent quinazoline derivative was shown to retain high affinity for, and act as a competitive...
A fluorescent quinazoline derivative was shown to retain high affinity for, and act as a competitive...
Background and purpose: Pharmacological analysis of synergism or functional antagonism between diffe...
Cellular distribution and binding characteristics of native alpha(1)-adrenoceptors (ARs) were determ...
The vascular endothelial growth factor (VEGF) family of cytokines are key drivers of blood vessel gr...
Fluorescent ligands provide the means of studying receptors in whole tissues using confocal laser sc...
Fluorescent ligands provide the means of studying receptors in whole tissues using confocal laser sc...
There is a need to develop new techniques for quantitative measurement of receptors expression on pa...
This review is based on the JR Vane Medal Lecture presented at the BPS Winter Meeting in December 20...
A better understanding of the mechanisms governing receptor trafficking between the plasma membrane ...
There is a need to develop new techniques for quantitative measurement of receptors expression on pa...
This chapter reviews and compares the strengths, limitations, and potential for development of the t...
1: This study examines the cellular localization of α1-adrenoceptors and demonstrates that binding t...
The discovery of beta-adrenoceptors in previously unsuspected cell types is contributing to the reth...
Background and purpose: Pharmacological analysis of synergism or functional antagonism between diffe...
A fluorescent quinazoline derivative was shown to retain high affinity for, and act as a competitive...
A fluorescent quinazoline derivative was shown to retain high affinity for, and act as a competitive...
Background and purpose: Pharmacological analysis of synergism or functional antagonism between diffe...
Cellular distribution and binding characteristics of native alpha(1)-adrenoceptors (ARs) were determ...
The vascular endothelial growth factor (VEGF) family of cytokines are key drivers of blood vessel gr...
Fluorescent ligands provide the means of studying receptors in whole tissues using confocal laser sc...
Fluorescent ligands provide the means of studying receptors in whole tissues using confocal laser sc...
There is a need to develop new techniques for quantitative measurement of receptors expression on pa...
This review is based on the JR Vane Medal Lecture presented at the BPS Winter Meeting in December 20...
A better understanding of the mechanisms governing receptor trafficking between the plasma membrane ...
There is a need to develop new techniques for quantitative measurement of receptors expression on pa...