Modular delivery of CpG-incorporated lipid-DNA nanoparticles for spleen DC activation
- Jin, Jun-O
- Park, Haein
- Zhang, Wei
- de Vries, Jan Willem
- Gruszka, Agnieszka
- Lee, Myung Won
- Ahn, Dae-Ro
- Herrmann, Andreas
- Kwak, Minseok
DOIAbstract
We introduce a versatile carrier system for in vitro and in vivo immune stimulation based on soft matter DNA nanoparticles (NPs). The incorporation of lipid-modified nucleotides into DNA strands enables the formation of micelles of uniform size. In a single self-assembly step, the micelles can be equipped with immune adjuvant (CpG) motifs and fluorescent probes. The immunological effects of CpG confined at the NP surface were studied in a comprehensive manner in animal experiments. Dose-dependent activation of spleen dendritic cells (DCs) by CpG-conjugated NP was observed, which was accompanied by the pronounced up-regulation of co-stimulatory molecule and cytokine production. (C) 2016 Elsevier Ltd. All rights reserved.</p
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