The mycobacterial PQS dioxygenase AqdC, a cofactor-less protein with an α/β-hydrolase fold, inactivates the virulence-associated quorum-sensing signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS) produced by the opportunistic pathogen Pseudomonas aeruginosa and is therefore a potential anti-virulence tool. We have used computational library design to predict stabilizing amino acid replacements in AqdC. While 57 out of 91 tested single substitutions throughout the protein led to stabilization, as judged by increases in (Formula presented.) of >2 °C, they all impaired catalytic activity. Combining substitutions, the proteins AqdC-G40K-A134L-G220D-Y238W and AqdC-G40K-G220D-Y238W showed extended half-lives and the best trade-off between...
In Pseudomonas aeruginosa, quorum sensing (QS) regulates the production of secondary metabolites, ma...
The study by Dulcey and colleagues in this issue of Chemistry & Biology changes our perception of th...
Pseudomonas aeruginosa represents a significant threat to public health. P. aeruginosa is a ubiquito...
The mycobacterial PQS dioxygenase AqdC, a cofactor-less protein with an α/β-hydrolase fold, inactiva...
The opportunistic pathogen Pseudomonas aeruginosa employs quorum sensing to govern the production of...
The opportunistic pathogen Pseudomonas aeruginosa employs quorum sensing to govern the production of...
Pseudomonas aeruginosa produces a number of alkylquinolone-type secondary metabolites best known for...
The opportunistic pathogen Pseudomonas aeruginosa employs quorum sensing to govern the production of...
Summary2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) is a quorum-sensing signal molecule used by Pseudomo...
The 2-alkyl-3-hydroxy-4(1H)-quinolone 2,4-dioxygenase HodC was previously described to cleave the Ps...
The rapid emergence of resistant bacteria is arising and causing a global health crisis. Antibiotic ...
<div><p><i>Pseudomonas aeruginosa</i> is an important opportunistic human pathogen that can establis...
Antibiotic crisis – Back to the startA famous quote of Charles Darwin reads: “It is not the stronges...
Bacterial populations co-ordinate gene expression collectively through quorum sensing (QS), a cell-t...
The pqs quorum sensing (QS) system is crucial for Pseudomonas aeruginosa virulence both in vitro and...
In Pseudomonas aeruginosa, quorum sensing (QS) regulates the production of secondary metabolites, ma...
The study by Dulcey and colleagues in this issue of Chemistry & Biology changes our perception of th...
Pseudomonas aeruginosa represents a significant threat to public health. P. aeruginosa is a ubiquito...
The mycobacterial PQS dioxygenase AqdC, a cofactor-less protein with an α/β-hydrolase fold, inactiva...
The opportunistic pathogen Pseudomonas aeruginosa employs quorum sensing to govern the production of...
The opportunistic pathogen Pseudomonas aeruginosa employs quorum sensing to govern the production of...
Pseudomonas aeruginosa produces a number of alkylquinolone-type secondary metabolites best known for...
The opportunistic pathogen Pseudomonas aeruginosa employs quorum sensing to govern the production of...
Summary2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) is a quorum-sensing signal molecule used by Pseudomo...
The 2-alkyl-3-hydroxy-4(1H)-quinolone 2,4-dioxygenase HodC was previously described to cleave the Ps...
The rapid emergence of resistant bacteria is arising and causing a global health crisis. Antibiotic ...
<div><p><i>Pseudomonas aeruginosa</i> is an important opportunistic human pathogen that can establis...
Antibiotic crisis – Back to the startA famous quote of Charles Darwin reads: “It is not the stronges...
Bacterial populations co-ordinate gene expression collectively through quorum sensing (QS), a cell-t...
The pqs quorum sensing (QS) system is crucial for Pseudomonas aeruginosa virulence both in vitro and...
In Pseudomonas aeruginosa, quorum sensing (QS) regulates the production of secondary metabolites, ma...
The study by Dulcey and colleagues in this issue of Chemistry & Biology changes our perception of th...
Pseudomonas aeruginosa represents a significant threat to public health. P. aeruginosa is a ubiquito...