Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of beta-Glucosidase:Synthesis, Characterization, and Biocatalytic Properties
- Orfanakis, Georgios
- Patila, Michaela
- Catzikonstantinou, Alexandra V.
- Lyra, Kyriaki-Marina
- Kouloumpis, Antonios
- Spyrou, Konstantinos
- Katapodis, Petros
- Paipetis, Alkiviadis
- Rudolf, Petra
- Gournis, Dimitrios
- Stamatis, Haralambos
DOIAbstract
Hybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of beta-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. The hybrid nanostructure-based biocatalysts formed exhibited a two to four-fold higher thermostability as compared to the free enzyme, as well as an enhanced performance at higher temperatures (up to 70 degrees C) and in a wider pH range. Moreover, these biocatalysts retained a significant part of their bioactivity (up to 40%) after 12 repeated reaction cycles.</p
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