Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining β-Exoglucosidases In Situ and In Vivo
- Kallemeijn, Wouter W.
- Li, Kah-Yee
- Witte, Martin D.
- Marques, André R.A.
- Aten, Jan
- Scheij, Saskia
- Jiang, Jianbing
- Willems, Lianne I.
- Voorn-Brouwer, Tineke M.
- Roomen, Cindy P.A.A. van
- Ottenhoff, Roelof
- Boot, Rolf G.
- Elst, Hans van den
- Walvoort, Marthe T.C.
- Florea, Bogdan I.
- Codée, Jeroen D.C.
- Marel, Gijsbert A. van der
- Aerts, Johannes M.F.G.
- Overkleeft, Herman S.
DOIAbstract
Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases. These probes offer new ways to study β-exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity-based probes targeting other retaining glycosidase families
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