Development and application of protein-based probes for correlated microscopy

Summarizing this thesis, the diversity is shown of protein labeling using nanobodies in different microscopic techniques. A new probe – FLIPPER-body – shows a efficient labeling in multiple microscopic techniques, in which multiple advantages are present compared to conventional antibodies. This small probe for correlated microscopy can become universal in use, because of the ease to adjust individual components towards researchers own wishes. Using this new method it was shown how extracellular vesicles can release their cargo to the cytoplasm. This proves that the new probe can contribute to the understanding of the building blocks in the human body