We investigate the relation between the parameters of the energy spectrum and the frequency and amplitude of the kilohertz quasi-periodic oscillations (kHz QPOs) in the low-mass X-ray binary 4U 1636-53. We fit the 3-180-keV spectrum of this source with a model that includes a thermal Comptonization component. We show that the frequencies of both kHz QPOs follow the same relation as a function of the parameters of this spectral component, except for a systematic frequency shift, whereas the rms fractional amplitude of each QPO follows a different relation with respect to those same parameters. This implies that, while the dynamical mechanism that sets the frequencies of the QPO can be the same for both kilohertz quasi-periodic oscillation (k...