In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes. Screening of the GenBank database resulted in the identification of a corresponding gene or EST for 490 of them. For 211 of the tags no homology could be detected. A major problem of the serial analysis of gene expression (SAGE) approach is how to further analyse the unknown tags. We have developed an RT-PCR-based method, rapid analysis of unknown SAGE tags (RAST-PCR), to analyse the expression of the corresponding genes. This approach can be used as a screening method to investigate whether or not the gene is differentially expressed between several cell types of interest.</p
The serial analysis of gene expression (SAGE) method is based on the isolation of unique sequence ta...
The SAGE (serial analysis of gene expression) method is sensitive at detecting the lower abundance t...
Serial analysis of gene expression (SAGE) is a powerful technique that can be used for global analys...
In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes....
In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes....
Serial analysis of gene expression (SAGE) is a powerful technique that can be used for global analys...
Serial analysis of gene expression, or SAGE, is an experimental technique designed to gain a direct ...
Serial analysis of gene expression (SAGE™) is a patented, large-scale mRNA-profiling technology that...
Serial analysis of gene expression (SAGE) is a powerful tool that allows digital analysis of overall...
Tumor markers and antigens are normally highly expressed in malignant tissue, but not in the surroun...
Serial analysis of gene expression (SAGE) is a sequencing-based technique that permits the simultane...
Tumor markers and antigens are normally highly expressed in malignant tissue, but not in the surroun...
Serial Analysis of Gene Expression (SAGE) is a technique that allows genome-wide expression studies ...
AbstractSAGE (serial analysis of gene expression) detects transcripts by extracting short tags from ...
Serial analysis of gene expression (SAGE) is a powerful tool, which provides quantitative and compre...
The serial analysis of gene expression (SAGE) method is based on the isolation of unique sequence ta...
The SAGE (serial analysis of gene expression) method is sensitive at detecting the lower abundance t...
Serial analysis of gene expression (SAGE) is a powerful technique that can be used for global analys...
In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes....
In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes....
Serial analysis of gene expression (SAGE) is a powerful technique that can be used for global analys...
Serial analysis of gene expression, or SAGE, is an experimental technique designed to gain a direct ...
Serial analysis of gene expression (SAGE™) is a patented, large-scale mRNA-profiling technology that...
Serial analysis of gene expression (SAGE) is a powerful tool that allows digital analysis of overall...
Tumor markers and antigens are normally highly expressed in malignant tissue, but not in the surroun...
Serial analysis of gene expression (SAGE) is a sequencing-based technique that permits the simultane...
Tumor markers and antigens are normally highly expressed in malignant tissue, but not in the surroun...
Serial Analysis of Gene Expression (SAGE) is a technique that allows genome-wide expression studies ...
AbstractSAGE (serial analysis of gene expression) detects transcripts by extracting short tags from ...
Serial analysis of gene expression (SAGE) is a powerful tool, which provides quantitative and compre...
The serial analysis of gene expression (SAGE) method is based on the isolation of unique sequence ta...
The SAGE (serial analysis of gene expression) method is sensitive at detecting the lower abundance t...
Serial analysis of gene expression (SAGE) is a powerful technique that can be used for global analys...