Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli

The SecY protein and other membrane proteins of Escherchia coli were solubilized by mixed micelles of n-octyl β-D-glucopyranoside, phospholipids, and glycerol. Proteoliposomes formed from this extract by detergent dialysis supported energy-dependent translocation and processing of pro-OmpA. Translocation required ATP, SecY, and SecA and was stimulated by a proton-motive force. These results provide an important assay for the isolation and identification of membrane components involved in protein translocation