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Crystallization and Preliminary Crystallographic Characterization of Endo-polygalacturonase II from Aspergillus niger

  • Schröter, K.-H.
  • Arkema, A.
  • Kester, H.C.M.
  • Visser, J.
  • Dijkstra, B.W.
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Publication date
October 1994
Icon DOI
10.1006/jmbi.1994.1660

Abstract

The endo-polygalacturonase II from Aspergillus niger has been crystallized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P2(1), with cell dimensions a = 60.6 Angstrom, b = 152.6 Angstrom, c = 74.0 Angstrom and beta = 91.2 degrees with four molecules per asymmetric unit. The crystals diffract to at least 2.8 Angstrom resolution and are suitable for X-ray analysis.</p

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