Investigation of structural, optical and antibacterial effect of copper nanoparticles synthesis by photoreduction

The copper nanoparticles films has been prepared via the reduction of copper salts CuSO4•5H2O by UV-lamps. The irradiation time changed from 15 min. to one hour effects on the morphology of dispersed copper nanoparticles were studied. The structure properties of the films were determined by X-ray diffraction, the change on the surface morphology was observed using atomic force microscope (AFM). Finally, The optical transmission of thin films was measured by UV-VIS spectrometer, during the preparation of observed that the color of dispersion gradually changed from blue coloration, brown finally dark brown by changing with irradiation time .As well as studies the biochemical and antibacterial effect of nano colloid studying by using Pseudomonas aeruginosa, Bacillus subtitis, Escherichia coli and Klebsiella phenmoniae. The copper nanoparticles is increasing its antibacterial activities. Actions of copper nanoparticles synthesized in both above method was also observed against both gram (-) and gram (+) bacteria