Immune performances of sangkuriang catfish (Clarias gariepinus) with addition of potassium diformate on feed

This research aims to determine the effective dosage of potassium diformate (KDF) in feed to enhance sangkuriang catfish immune performances. The research was conducted on November - January 2019, at Aquaculture Laboratory Building No. 4 Faculty of Fisheries and Marine Sciences Universitas Padjadjaran. This study used an experimental method of Completely Randomized Design (CRD), with four treatments and three replications. The treatments are the addition of potassium diformate into feed with doses A (0%), B (0.5%), C (0.7%) and D (0.9%). Observed parameters are clinical symptoms, total leukocyte count, total erythrocyte count, specific growth rate, survival rate and water quality. The results showed that 0.5% KDF feed produced the highest survival rate of 85% and the highest specific growth rate of 3.59% after 40 days. Furthermore, due to treatment, total erythrocyte count increased from 2.09×106 cells/mm3, to 2.10×106 cells/mm3 (8.1%) and total leukocyte count increased from 7.1×104 cells/mm3, to 10.7×104 cells/mm3 (42.81%). The addition of 0.5% KDF in feed is effective in improving the immune performances of sangkuriang catfish challenged by A. hydrophila, with the highest survival rate of 97%. Moreover, recovery of total leukocyte count and total erythrocyte count was faster than other treatments as of day 7th, with a value 1.97×106 sel/mm3and 12.60×104 sel/mm3. It also resulted in the lowest gross clinical signs and the fastest recovering