Effectiveness of potassium diformate addition to feed to improve immune system of Pangasius (Pangasianodon hypophthalmus) that challenged by Aeromonas hydrophila

This research was conducted from October 2018 until April 2019 in the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. This study aims to determine the optimum potassium diformate dosage which was added to feed to increase the immune status of Pangasianodon hypophthalmus (Sauvage, 1878). The research method used is the experimental Complete Random Design (CRD) with 4 treatments and 3 replications. The treatments are A (control), B (0.3% KDF), C (0.5% KDF), and D (0.7% KDF). Observed parameters are survival rate, gross clinical signs, intestine pH levels, total leukocyte count (white blood cells), total erythrocyte count (red blood cells), and water quality. The results showed that the 0.5% KDF addition in feed is the most effective to improve immune status. This treatment also is the most effective to improve Pangasius immune status after being challenged with A. hydrophila with survival rate of 93.33%, leukocyte count 2.98×106 cells/mm3 and erythrocyte count 1.02×106 cells/mm3. Increased leukocyte count was 65.26% and erythrocyte count - 58.23%