The usefulness of evaluating serum immunoglobulin light free chains concentration in diagnosis of patients with plasma cell disorder

Patients with plasma cell disorder require a medical examination that will enable in a quick way to make a diagnosis, treatment selection and conduct patient monitoring during drug therapy. The aim of this study was to determine the concentration of serum immunoglobulin and light free chains in patients with monoclonal gammopathy. The blood serum of patients was evaluated in the Beskid Hematological Clinic Cancer Center – City Hospital in Bielsko-Biała. The serum of immunoglobulin light free chains was determined by using commercially available tests by the Binding Site company. Assay validation was performed in the serum light free chains of immunoglobulin by setting the analytical range of the method, linearity, precision, analytical sensitivity, recurrence and limit of detection as well as quantification methods. The results obtained indicate that the determination of serum immunoglobulin and light free chains is important in the diagnosis of patients with multiple myeloma and other monoclonal gammopathy, such as smouldering myeloma, light chain disease, gammopathy of undetermined significance, non-Hodgkin lymphoma and amyloidosis