Developing and democratizing tools for spatially resolved profiling of genome and transcriptome

The common thread of the work presented in this thesis is the development and democratization of tools that facilitate the spatial profiling of genome and transcriptome. Paper I describes iFISH, a resource that facilitates the large-scale design and costeffective, flexible production of probes for DNA FISH. More specifically, we created a database of oligos targeting the human genome and a probe design tool which we made publicly available through a user-friendly web interface, which we have named iFISH4U. We used iFISH4U to design hundreds of small probes (