Biosurveillance for invasive xylophagous beetles using a DNA metabarcoding approach

International audienceInvasive xylophagous beetles represent a threat to forests worldwide. There is an urgent need to develop a biosurveillance system for early detection of invasive species. We have used multi-component blends for mass trapping both native and exotic longhorn beetles at several European ports of entry. With this biological material at our disposal, we are developing reference databases of Cerambycides in order to improve the number of species detectable in metabarcoding analyses. We are also working on the design of new primers to improve the detectability rate of these species. Finally, we are developing a non-destructive DNA extraction method for multiple insects at the same time allowing the conservation of all extracted samples