Add to ORKGA detailed quantification of antigen processing by endosomal compartments provides important information on the pattern of protein fragmentation. Here, we describe a protocol that combines gradient purified endosomes, incubated with antigens, followed by hot spot analysis of MS/MS-sequenced peptides. The analysis identifies differences in endosomal antigen processing by dendritic cells under diverse experimental conditions. For complete details on the use and execution of this protocol, please refer to Clement et al. (2021)
Mass spectrometry (MS)-based methods coupled to reverse phase chromatography separation are a useful...
<p>A. Vn96 peptides precipitate vesicular structures from conditioned cell culture media. The biotin...
We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extrac...
Summary: A detailed quantification of antigen processing by endosomal compartments provides importan...
The present thesis looks at the behaviour of internalised cell surface-derived membrane marker in co...
<span>Endolysosomal processing has a critical influence on immunogenicity as well as immune polariza...
Extracellular DNA is engulfed by innate immune cells and digested by endosomal DNase II to generate ...
Dendritic cells (DCs) take up soluble- or cell-associated antigens and digest them, delivering fragm...
Background: The growing number of novel candidate molecules for the treatment of allergic diseases i...
Major histocompatibility complex class I (MHC-1) molecules bind peptide fragments derived from cytos...
Endolysosomal processing has a critical influence on immunogenicity as well as immune polarization o...
Antigen processing for presentation by antigen presenting cells (APCs) expressing class II major his...
Dendritic cells (DCs) are specialized leukocytes that orchestrate the adaptive immune response. Mass...
Endocytosis is one of the most dynamic processes in a mammalian cell. Beside the regulation of prote...
Presentation of peptides on major histocompatibility complex class I (MHC I) is essential for the es...
Mass spectrometry (MS)-based methods coupled to reverse phase chromatography separation are a useful...
<p>A. Vn96 peptides precipitate vesicular structures from conditioned cell culture media. The biotin...
We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extrac...
Summary: A detailed quantification of antigen processing by endosomal compartments provides importan...
The present thesis looks at the behaviour of internalised cell surface-derived membrane marker in co...
<span>Endolysosomal processing has a critical influence on immunogenicity as well as immune polariza...
Extracellular DNA is engulfed by innate immune cells and digested by endosomal DNase II to generate ...
Dendritic cells (DCs) take up soluble- or cell-associated antigens and digest them, delivering fragm...
Background: The growing number of novel candidate molecules for the treatment of allergic diseases i...
Major histocompatibility complex class I (MHC-1) molecules bind peptide fragments derived from cytos...
Endolysosomal processing has a critical influence on immunogenicity as well as immune polarization o...
Antigen processing for presentation by antigen presenting cells (APCs) expressing class II major his...
Dendritic cells (DCs) are specialized leukocytes that orchestrate the adaptive immune response. Mass...
Endocytosis is one of the most dynamic processes in a mammalian cell. Beside the regulation of prote...
Presentation of peptides on major histocompatibility complex class I (MHC I) is essential for the es...
Mass spectrometry (MS)-based methods coupled to reverse phase chromatography separation are a useful...
<p>A. Vn96 peptides precipitate vesicular structures from conditioned cell culture media. The biotin...
We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extrac...