In plants, calcium signals are involved in multiple physiologicalprocesses such as stomatal opening, stress responses, and polarized growth ofroot hairs and pollen tubes. These signals are given as repetitive oscillationsof cytosolic free Ca2+ where the intensity and amplitude correlateaccording to the stimulus. Pollen tube growth occurs through the concertedaction of different factors such as pH, ROS, actin and calcium gradient. Anyimbalance between these factors causes aborted pollen tubes and therefore, defectsin fertility.In this work, we propose to perform a functional study of the P2B typecalcium pumps (Autoinhibited Ca2+-ATPases,ACAs), in pollen tubes of Arabidopsis thaliana. These pumps are mainlylocated in vacuole, endoplasmic reti...