Participation of transcription factors from the Rel/NF-κB family in the circadian system in hamsters

Marpegan, Luciano
Bekinschtein, Tristán Andrés
Freudenthal, Ramiro A. M.
Rubio, Maria Fernanda
Ferreyra, Gabriela
Romano, Arturo Gabriel
Golombek, Diego Andrés

Open link

Publication date

March 2004

DOI

10.1016/j.neulet.2003.12.112

Publisher

Elsevier BV

Journal

Neuroscience Letters

Abstract

We have studied the presence and activity of components of the nuclear factor-κB (NF-κB) transcription factor in the hamster circadian system analyzing wheel-running activity, protein expression and DNA binding activity by electrophoresis mobility shift assays (EMSA). Non-rhythmic specific immunoreactive bands corresponding to a NF-κB subunit (p65) were found in hamster suprachiasmatic nuclei (SCN) homogenates. The active form of NF-κB evidenced by EMSA was clear and specific in SCN nuclear extracts. The administration of the NF-κB inhibitor pyrrolidine-dithiocharbamate (PDTC) blocked the light-induced phase advance at circadian time 18 (vehicle+light pulse: 2.08±0.46 h, PDTC+light: 0.36±0.35 h). These results demonstrate the presence and a...