Microbial characterization of a facultative residual sludge obtained from a biogas plant with ability to degrade commercial B10 diesel oil

Biodegradation of commercial B10 diesel oil (DB10) by the microbial consortium present in a biogas residual sludge was first investigated. Maximum removal efficiencies of the petrochemical and fatty acids methyl ester (FAME) fractions of DB10 were 55 ± 3% and 94 ± 6%, respectively. Taxonomic profiling of the microbial consortium present in the residual sludge was carried out in order to identify potential hydrocarbon-degrading bacteria. After mild selection pressure using DB10, three Pseudomonas species (P. aeruginosa, P. stutzeri and P. mendocina) were mostly found in the treated sample. However, only P. aeruginosa and P. stutzeri showed a rapid growth and high dehydrogenase activity at the expense of DB10 as the sole carbon source. In addition, both strains removed 42 ± 5% and 53 ± 2% of the petrochemical fraction, and >75% of the FAME fraction, respectively. Similar results were found with a bacterial consortium of the three isolated Pseudomonas, indicating no synergistic hydrocarbon degradation by these strains. As expected, biosurfactant production by the pseudomonads was directly associated with the bacterial DB10 biodegradation performance. These results are the first describing petroleum-based hydrocarbon biodegradation ability of a crude facultative residual sludge obtained from a biogas facility; and represent a rational first step in order to understand which bacteria in the sludge may act in petroleum-based fuels degradation.Fil: Loureiro, Dana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Católica de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Olivera, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Católica de Rosario; ArgentinaFil: Tondo, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Universidad Católica de Rosario; ArgentinaFil: Herrero, María Sol. Universidad Católica de Rosario; ArgentinaFil: Salvatierra, Lucas Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Católica de Rosario; ArgentinaFil: Perez, Leonardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Universidad Católica de Rosario; Argentin