Oxygen Affinities and Electrophoretic Patterns of Hemoglobins in Trout and Basses from Virginia

Hemoglobin solutions were prepared from pooled samples of blood taken from each of the following species; Salmo gairdneri, the rainbow trout; Salvelinus fontinalis, the brook trout; Salmo trutta, the brown trout; Micropterus dolomieui, the smallmouth bass; Micropterus salmoides, the largemouth bass. Hemoglobin electrophoretic patterns for each species were developed on cellulose acetate membranes. Oxyhemoglobin affinity curves were determined spectrophotometrically on different hemoglobin solutions from each species. Interspecific differences concerned with hemoglobin electrophoretic patterns, oxyhemoglobin affinities, and the Bohr effect were shown for both trout and basses