Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells
- Faklaris, Orestis
- Joshi, Vandana
- Irinopoulou, Theano
- Tauc, Patrick
- Girard, Hugues
- Gesset, Celine
- Sennour, Mohamed
- Thorel, Alain
- Arnault, Jean-Charles
- Boudou, Jean-Paul
- Curmi, Patrick A.
- Treussart, François
DOIAbstract
International audienceDiamond nanoparticles (nanodiamonds) have been recently proposed as new labels for cellular imaging. For small nanodiamonds (size <40 nm) resonant laser scattering and Raman scattering cross-sections are too small to allow single nanoparticle observation. Nanodiamonds can however be rendered photoluminescent with a perfect photostability at room temperature. Such a remarkable property allows easier single-particle tracking over long time-scales. In this work we use photoluminescent nanodiamonds of size <50 nm for intracellular labeling and investigate the mechanism of their uptake by living cells . By blocking selectively different uptake processes we show that nanodiamonds enter cells mainly by endocytosis and converg...
Add to ORKG