Proliferation of human bone marrow stem cells cultured on alfa-tricalcium phosphate cement and titanium discs

Objectives: to evaluate human bone marrow stem cells (HBMSC) cultured on a-tricalcium phosphate cement (a-TCP) and titanium (Ti) discs, as well as the osteoconductive properties of these biomaterials. Methods: discs of a-TCP and commercially pure Ti were manufactured and placed on polyethylene plates. Cells were cultured according to the following: group I - control (no discs); group II - a-TCP discs; group III - Ti discs. Propidium iodide staining was used to assess cell proliferation. Cells cultures were analyzed at 3, 7, 14 and 21 days. Results: Proliferation occurred in both biomaterials. At 3 days, greater cell proliferation was seen in group II than group III (p=0.01). Differences were not significant on the other time periods. Morphologic analysis showed circular morphology in group I, compatible with osteogenic cell lineage. Conclusions: both biomaterials were osteoconductive but not osteoinductive. This did not affect the initial stages of osteogenesis.Objectives: to evaluate human bone marrow stem cells (HBMSC) cultured on α -tricalcium phosphate cement (α -TCP) and titanium (Ti) discs, as well as the osteoconductive properties of these biomaterials. Methods: discs of α -TCP and commercially pure Ti weremanufactured and placed on polyethylene plates. Cells were cultured according to the following: group I – control (no discs); group II – α-TCP discs; group III – Ti discs. Propidium iodide staining was used to assess cell proliferation. Cells cultures were analyzed at 3,7, 14 and 21 days. Results: Proliferation occurred in both biomaterials. At 3 days, greater cell proliferation was seen in group II than group III (p=0.01). Differences were not significant on the other time periods. Morphologic analysis showed circular morphology in group I, compatible with osteogenic cell lineage. Conclusions: both biomaterials were osteoconductive but not osteoinductive. This did not affect the initial stages of osteogenesis