Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase
- Rodrigues, Matthew
- Giri, Nitai
- Royant, Antoine
- Zhang, Yang
- Bolton, Rachel, Ann
- Evans, Gwyndaf
- Ealick, Steven E.
- Begley, Tadhg P.
- Tews, Ivo
DOIAbstract
The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I333 intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis
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