Synthetic protein-binding tools based on anti-green fluorescent protein (GFP) nanobodies have recently emerged as useful resources to study developmental biology. By fusing GFP-targeting nanobodies to well-characterized protein domains residing in discrete sub-cellular locations, it is possible to directly and acutely manipulate the localization of GFP-tagged proteins-of-interest in a predictable manner. Here, we describe a detailed protocol for the application of nanobody-based GFP-binding tools, namely Morphotrap and GrabFP, to study the localization and function of extracellular and intracellular proteins in the Drosophila wing imaginal disc. Given the generality of these methods, they are easily applicable for use in other tissues and m...
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switche...
Protein-protein interactions are crucial for cellular homeostasis and play important roles in the dy...
Protein–protein interactions are crucial for cellular homeostasis and play important roles in the dy...
The role of protein localization along the apical-basal axis of polarized cells is difficult to inve...
The direct manipulation of proteins by nanobodies and other protein binders has become an additional...
International audienceIn cell biology, detection of protein subcellular localizations is often achie...
We describe a technique to tag Drosophila proteins with GFP at their native genomic loci. This techn...
The Drosophila genome contains >13,000 protein coding genes, the majority of which remain poorly inv...
In cell biology, detection of protein subcellular localizations is often achieved by optical microsc...
Abstract Measuring the positions and dynamics of proteins in intact tissues or whole animals is key ...
Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein localization...
The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly inve...
Nanobodies are small, monomeric antibody mimetic proteins produced by members of the camelid family ...
Measuring the positions and dynamics of proteins in intact tissues or whole animals is key to unders...
Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization ...
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switche...
Protein-protein interactions are crucial for cellular homeostasis and play important roles in the dy...
Protein–protein interactions are crucial for cellular homeostasis and play important roles in the dy...
The role of protein localization along the apical-basal axis of polarized cells is difficult to inve...
The direct manipulation of proteins by nanobodies and other protein binders has become an additional...
International audienceIn cell biology, detection of protein subcellular localizations is often achie...
We describe a technique to tag Drosophila proteins with GFP at their native genomic loci. This techn...
The Drosophila genome contains >13,000 protein coding genes, the majority of which remain poorly inv...
In cell biology, detection of protein subcellular localizations is often achieved by optical microsc...
Abstract Measuring the positions and dynamics of proteins in intact tissues or whole animals is key ...
Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein localization...
The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly inve...
Nanobodies are small, monomeric antibody mimetic proteins produced by members of the camelid family ...
Measuring the positions and dynamics of proteins in intact tissues or whole animals is key to unders...
Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization ...
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switche...
Protein-protein interactions are crucial for cellular homeostasis and play important roles in the dy...
Protein–protein interactions are crucial for cellular homeostasis and play important roles in the dy...