Computer-based methods are excellent tools to modify existing proteins. The software suite Rosetta offers a large variety of options to solve many problems of protein design. During the last decades a steadily increasing number of protocols became available and more complex concepts for the modelling of proteins and their function arouse. Among them is multi-state protein design (MSD) that utilizes in parallel several three-dimensional conformations of a protein to increase the chances of a successful design. In the first part of this work I used MSD to reprogram a protein interface by means of an anchored design approach. The starting point was a pair of glutamine amidotransferase complexes consisting both of homologous pairs of synthase ...
The sheer size of the protein sequence space is massive: a protein of 100 residues can have 20^100 p...
Traditional computational protein design (CPD) calculations model sequence perturbations and evaluat...
Computational protein design has the ambitious goal of crafting novel proteins that address challeng...
Computer-based methods are excellent tools to modify existing proteins. The software suite Rosetta o...
<div><p>Computational protein design (CPD) is a powerful technique to engineer existing proteins or ...
Most successes to date in computational protein design have relied on optimizing sequences to fit we...
The RosettaDesign server identifies low energy amino acid sequences for target protein structures ()...
Computational modeling is a powerful companion to direct experimental testing, because it allows res...
Protein design is a procedure for computing natural-like sequences that will fold into a specifiedst...
This dissertation describes the development, benchmarking, validation, and application of computatio...
Computational protein design aims to predict protein sequences that will fold into a given three-dim...
Some protein design tasks cannot be modeled by the traditional single state design strategy of findi...
Proteins are typically represented by discrete atomic coordinates providing an accessible framework ...
Proteins propagate information within a cell by interacting with other proteins, DNA, RNA, and small...
Finely orchestrated protein-protein interactions are at the heart of virtually all fundamental cellu...
The sheer size of the protein sequence space is massive: a protein of 100 residues can have 20^100 p...
Traditional computational protein design (CPD) calculations model sequence perturbations and evaluat...
Computational protein design has the ambitious goal of crafting novel proteins that address challeng...
Computer-based methods are excellent tools to modify existing proteins. The software suite Rosetta o...
<div><p>Computational protein design (CPD) is a powerful technique to engineer existing proteins or ...
Most successes to date in computational protein design have relied on optimizing sequences to fit we...
The RosettaDesign server identifies low energy amino acid sequences for target protein structures ()...
Computational modeling is a powerful companion to direct experimental testing, because it allows res...
Protein design is a procedure for computing natural-like sequences that will fold into a specifiedst...
This dissertation describes the development, benchmarking, validation, and application of computatio...
Computational protein design aims to predict protein sequences that will fold into a given three-dim...
Some protein design tasks cannot be modeled by the traditional single state design strategy of findi...
Proteins are typically represented by discrete atomic coordinates providing an accessible framework ...
Proteins propagate information within a cell by interacting with other proteins, DNA, RNA, and small...
Finely orchestrated protein-protein interactions are at the heart of virtually all fundamental cellu...
The sheer size of the protein sequence space is massive: a protein of 100 residues can have 20^100 p...
Traditional computational protein design (CPD) calculations model sequence perturbations and evaluat...
Computational protein design has the ambitious goal of crafting novel proteins that address challeng...