Evaluation of fluoride uptake in the enamel of primary teeth after using different concentrations of a green tea extract: An in-vitro study

It is believed that extracts of green tea increase fluoride uptake in deciduous enamel and the aim of this in vitro study was to evaluate the fluoride uptake in the enamel of primary teeth after using different concentrations of an extract of green tea. Seventy-five enamel samples were prepared from deciduous canine teeth of children in Kerman, Iran, and randomly divided into five groups of 15: Group 1 (G1): no treatment (negative control); Groups 2 (G2), 3 (G3), 4 (G4), and 5 (G5) were treated with green tea extract in concentrations of 0.2, 1, 2, and 10, respectively. After the surface treatment, all the samples were submitted to an acid challenge consisting of daily immersion for 5 days in a demineralizing (3 hr) and a mineralizing solution (21 hr). The fluoride concentrations were then calculated with a potentiometer. One-way ANOVA was used for statistical analyses (p<0.05). There was a significant difference between the fluoride uptake in the control and all other groups except for the G2 group (0.2 green tea extract) (p