Correlation Between Biochemical And Spermatological Parameters In Rainbow Trout (Oncorhynchus Mykiss) Semen

Levels of biochemical parameters in seminal plasma and physical parameters of rainbow trout (Oncorhynchus mykiss) semen were determined and compared. Seminal plasma contained 80.51±31.48 mmol/l Na+, 46.21±12.58 mmol/l K+, 4.65±1.57 mg/dl Ca2+, 3.48±1.18 mEq/l Mg2+, 112.5±25.93 mmol/l Cl-, 1.33±0.76 mg/dl glucose, 0.15±0.09 g/dl protein, 2.55±2.47 mg/dl cho- lesterol, 8.0±2.84 mg/dl triglyceride, and 31.65±40.78 mg/dl urea. Semen volume was 19.9±21.43 ml, spermatozoa motility 60.0±30.09%, duration of spermatozoa movement 174.0±1.82 s, spermatozoa concentration 1.52±0.70 x 109/ml, total spermatozoa number 44.25±64.59 x 109, and pH 6.7±0.25. There were significant positive correlations between semen volume and Na+ (r = 0.667, p<0.05), total spermatozoa number and semen volume (r = 0.977, p<0.01), total spermatozoa number and spermatozoa concentration (r = 0.652, p<0.05), Na+ and Cl- (r = 0.733, p<0.05), Na+ and triglyceride (r = 0.632, p<0.05), K+ and protein (r = 0.728, p<0.05), and Ca2+ and protein (r = 0.685, p<0.05). Significant negative correlations were found between Cl- and glucose (r = - 1.00, p<0.05), and triglyceride and urea (r = -0.716, p