Leptin is immunosuppressive in both Concanavalin A and Lipopolysaccharide mitogenesis of murine splenocytes in vitro : an honors thesis (HONRS 499)

The aim of this study was to determine the effect of recombinant murine leptin on the mitogen response mouse lymphocytes ill vitro. Mouse splenocytes were cultured from young mice and lymphocyte proliferation was measured in the presence of media, Con A, or LPS either in the presence or absence of leptin. This proliferation was measured using MTT uptake or 3H-thymidine uptake. Proliferation was then measured when leptin is present in the cell culture.We first established the optimum concentration of lymphocytes and the two mitogens in cell culture. We then added leptin at various concentrations under these optimized conditions and examined the effect which this hormone had on the proliferation of murine T or B cells. Our findings indicate that leptin iu vitro is immunosuppressive and has a dose-dependent negative effect on proliferation of both T and B cells in response to the specific T cell mitogen Con A and the B cell mitogen LPS.Honors CollegeThesis (B.?.