Add to ORKGA chitinolytic bacterium of the genus Pseudomonas, isolated from a pond, was grown in liquid culture. Culture filtrates were subjected to purification procedures. The extracellular material was found to contain chitinase and chitobiase activity. The chitinase activity was isolated from contaminating proteins by ammonium sulfate fractionation, affinity chromatography, and molecular weight exclusion chromatography. Overall purification was found to be three-fold, with a recovery of fourteen percent of the `original activity. Polyacrylamide gel electrophoresis showed the presence of two proteins, both exhibiting chitinase activity.Department of BiologyThesis (M.S.
274-278Chitinases, through decomposition of chitin, have wide applications, and hence are in demand....
The purpose of this study was to obtain superior chitinolytic bacteria capable of degrading chitin ...
The aim of this work was to find a bacterial catabolic enzyme, suitable for the deacetylation of chi...
The gram-negative bacterium Pseudomonas aeruginosa secretes many proteins into its extracellular env...
Chitin is the second most abundant natural biopolymer that is found in exoskeletons of crustaceans, ...
[[abstract]]The chitinase-producing strain TKU008 was isolated from soil in Taiwan, and it was ident...
The gram-negative bacterium Pseudomonas aeruginosa secretes many proteins into its extracellular env...
SUMMARY: A method for the detection of small quantities of chitinase in culture fluids is described ...
One hundred and twenty isolates of chitosanase producing bacteria were screened from water and soil ...
Chitinolytic bacteria were isolated from several exotic area in Manado Province. The most potential ...
ABSTRACT Chitinase is enzyme that hydrolyzes chitin, a polimer of P-1,4-N-Acetilglucosamine which is...
Chitinolytic bacteria were isolated from several exotic area in Manado Province. The most potential ...
The aim of this work was to find a bacterial catabolic enzyme, suitable for the deacetylation of chi...
This study aimed to determine the ability of bacteria to produce the chitinase enzyme, purify, and c...
[[abstract]]Two extracellular chitinases (FI and FII) were purified from the culture supernatant of ...
274-278Chitinases, through decomposition of chitin, have wide applications, and hence are in demand....
The purpose of this study was to obtain superior chitinolytic bacteria capable of degrading chitin ...
The aim of this work was to find a bacterial catabolic enzyme, suitable for the deacetylation of chi...
The gram-negative bacterium Pseudomonas aeruginosa secretes many proteins into its extracellular env...
Chitin is the second most abundant natural biopolymer that is found in exoskeletons of crustaceans, ...
[[abstract]]The chitinase-producing strain TKU008 was isolated from soil in Taiwan, and it was ident...
The gram-negative bacterium Pseudomonas aeruginosa secretes many proteins into its extracellular env...
SUMMARY: A method for the detection of small quantities of chitinase in culture fluids is described ...
One hundred and twenty isolates of chitosanase producing bacteria were screened from water and soil ...
Chitinolytic bacteria were isolated from several exotic area in Manado Province. The most potential ...
ABSTRACT Chitinase is enzyme that hydrolyzes chitin, a polimer of P-1,4-N-Acetilglucosamine which is...
Chitinolytic bacteria were isolated from several exotic area in Manado Province. The most potential ...
The aim of this work was to find a bacterial catabolic enzyme, suitable for the deacetylation of chi...
This study aimed to determine the ability of bacteria to produce the chitinase enzyme, purify, and c...
[[abstract]]Two extracellular chitinases (FI and FII) were purified from the culture supernatant of ...
274-278Chitinases, through decomposition of chitin, have wide applications, and hence are in demand....
The purpose of this study was to obtain superior chitinolytic bacteria capable of degrading chitin ...
The aim of this work was to find a bacterial catabolic enzyme, suitable for the deacetylation of chi...