In Vito Fertilization in Buffaloes: A Review

This is the review of original data concerning the effect of some factors on oocyte development in vitro of buffaloes. In vitro fertilization is a multi - step process: oocytes maturation, fertilization and embryo culture. In vitro fertilization is strongly influenced by events occurring during oocyte maturation, fertilization and the subsequent development of the fertilized oocytes. With the advancement of IVF procedures, variability in developmental rate and viability of in vitro produced buffalo embryos so, improving the efficiency and identifying the sources of variations between IVF systems are more important when routinely producing blastocysts from individuals of high genetic merits. Also, the development of specific culture regimes capable of supporting in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) to the blastocyst stage is highly desirable in breeding systems. This paper discusses the technical aspects of the procedures involved in in vitro fertilization of buffaloes