Water extracts from winery by-products as tobacco defense inducers

EcotoxicologyISI Document Delivery No.: AP6TNTimes Cited: 1Cited Reference Count: 31Benouaret, Razik Goujon, Eric Trivella, Aurelien Richard, Claire Ledoigt, Gerard Joubert, Jean-Marie Mery-Bernardon, Aude Goupil, PascaleFUI: Fonds Unique InterministerielThis work had financial support provided by grants from FUI: Fonds Unique Interministeriel. We thank Celine Sac for her help in growing tobacco plants and Dominique Marcon for the tobacco leaf images.SpringerDordrechtWater extracts from winery by-products exhibited significant plant defense inducer properties. Experiments were conducted on three marc extracts containing various amounts of polyphenols and anthocyanins. Infiltration of red, white and seed grape marc extracts into tobacco leaves induced hypersensitive reaction-like lesions with cell death evidenced by Evans Blue staining. The infiltration zones and the surrounding areas revealed accumulation of autofluorescent compounds under UV light. Leaf infiltration of the three winery by-product extracts induced defense gene expression. The antimicrobial PR1, beta-1,3-glucanase PR2, and chitinase PR3 target genes were upregulated locally in tobacco plants following grape marc extract treatments. The osmotin PR5 transcripts accumulated as well in red marc extract treated-tobacco leaves. Overall, the winery by-product extracts elicited an array of plant defense responses making the grape residues a potential use of high value compounds