Total polyphenol content and antioxidant activity of dietary supplements used by women in menopause

U posljednje vrijeme provode se mnoga istraživanja radi procjene antioksidativnog djelovanja polifenolnih sastavnica u hrani i dodacima prehrani. Ove biološki aktivne tvari su iznimno cijenjene zbog preventivnog i terapijskog djelovanja na ljudsko zdravlje. Cilj ovog rada bio je procijeniti kvalitetu dodataka prehrani koji sadrže lan, crvenu djetelinu, hmelj i konopljiku obzirom na ukupni sadržaj polifenola te odrediti antioksidativnu aktivnost istih. Sadržaj ukupnih polifenola u analiziranim dodacima prehrani određen je Folin-Ciocalteu metodom, dok je antioksidativna aktivnost istih procijenjena HPLC-DPPH metodom. Ova kromatografska metoda se pokazala brza i ponovljiva te prikladna za rutinsku analizu uzoraka. Ispitivani dodaci prehrani koji sadrže ekstrakte navedenih biljaka dobar su izvor polifenolnih spojeva. Antioksidativna aktivnost analiziranih dodataka prehrani u skladu je sa sadržajem ovih sekundarnih metabolita. Korelacija između ukupnog sadržaja polifenola i antioksidativne aktivnosti je statistički značajna, iz čega je vidljivo da je antioksidativno djelovanje proporcionalno koncentraciji ovih bioaktivnih sastavnica. Rezultati ovog istraživanja doprinose boljem razumijevanju sadržaja ukupnih polifenola i antioksidativne aktivnosti dodataka prehrani koji sadrže lan, crvenu djetelinu, hmelj i konopljiku.Recently, many studies have been conducted to evaluate the antioxidant activity of polyphenolic components in foods and dietary supplements. These biologically active substances are highly valued phytochemicals due to their preventive and therapeutic effects on human health. This study aimed to evaluate the quality of the flax-based, red clover-based, hop-based and chasteberry-based dietary supplements concerning the total polyphenol content and their antioxidant activity. The total polyphenol content in the analyzed dietary supplements was determined by Folin-Ciocalteu method, while the antioxidant activity was estimated by HPLC-DPPH method. This chromatography method has been shown to be fast and reproducible, and suitable for use in routine analysis. The tested dietary supplements containing extracts of these plants are a good source of polyphenolic compounds. The antioxidant activity of the analyzed dietary supplements is in accordance with the content of these secondary metabolites. The correlation between the total polyphenol content and antioxidant activity is statistically significant, and the antioxidant activity is proportional to the concentration of these bioactive compounds. The results of this study contribute to a better understanding of the content of total polyphenols and the antioxidant activity of flax-based, red clover-based, hop-based and chasteberry-based dietary supplements