Development of a new method for the detection and quantification of 3-nitrotyrosine

The nitration of Tyrosine residues in proteins is associated with nitrosative stress, resulting in the formation of 3-nitrotyrosine (3-NT). Different methods have been described for the quantification of this molecule. Regarding chromatographic methods, they seem to be very accurate, showing very good sensibility and specificity. Gas chromatography-based methods exhibit the highest sensibility, however a derivatization step prior analysis is required, which ends up being time-consuming for the analyst. Conversely, High Performance Liquid Chromatography (HPLC) may not require such derivatization.