Evaluation of four hemoglobin separation analyzers for hemoglobinopathy diagnosis

BackgroundFour automated hemoglobin separation devices are compared in their ability to detect hemoglobinopathies, both in HbA1c and in hemoglobinopathy mode. MethodsQuality control material and 58 samples, including one heterozygous -thalassemia sample, six heterozygote -thalassemia samples and 32 samples with a known hemoglobin variant, were used to assess imprecision of HbF and HbA2 measurements, correlation with the gold standard and sensitivity for detecting -thalassemia and Hb variants on D-100 (Bio-Rad Laboratories), HA 8180T (Menarini), HLC-723G8 (Tosoh Bioscience) and Capillarys 2 Flex Piercing (Sebia). ResultsImprecision was