Application of mineral-based amendments for enhancing phytostabilization in lolium perenne L. cultivation

An experimental investigation is conducted to explore the suitability of Lolium perenne L., diatomite, chalcedonite, dolomite, and limestone for the phytostabilization of Ni and Cu in contaminated soil. A controlled greenhouse study is conducted. The soil is enriched with rising dose of Cu and Ni, that is, (0, 150, 250, and 350 mg kg(-1)) and (0, 150, 300, and 450 mg kg(-1)), respectively. The phytostabilization potential of perennial ryegrass is evaluated using a bioaccumulation coefficient and translocation factor. Pseudo-total and available metal content (0.01M CaCl2) in soils and bioaccumulated content in plants are defined in laboratory experiments using spectrophotometry experimental technique. L. perenne is adequate in phytostabilization aided programs, simultaneously, diatomite, chalcedonite, dolomite, and limestone used as modifiers are effective in reducing the accessibility and mobility of metals in Cu-and Ni-polluted soils. The finding of the present study suggests that the studied element in the roots and above-ground parts of L. perenne differs significantly upon applying mineral-based modifications to the soil, synchronously the effect of increasing Cu and Ni levels. Application of dolomite and limestone to the soil cause the highest percentage of the above-ground biomass. Diatomite along with limestone cause a significant boost of Cu and Ni absorption in the roots. Limestone causes an increase in the contents of K, Na, and Ca, as well as a reduction in P in the above-ground parts of L. perenne. Limestone and chalcedonite leads to the highest decrease in available Cu and Ni