Aims. From accurate photometric and spectroscopic information, we build the Fundamental Plane (FP) relation for the early-type galaxies of the cluster Abell S1063. We use this relation to develop an improved strong lensing model of the cluster, and we decompose the cluster’s cumulative projected total mass profile into its stellar, hot gas, and dark matter mass components. We compare our results with the predictions of cosmological simulations. Methods. We calibrate the FP using Hubble Frontier Fields photometry and data from the Multi Unit Spectroscopic Explorer on the Very Large Telescope. The FP allows us to determine the velocity dispersions of all 222 cluster members included in the model from their measured structural parameters. A...