This dissertation describes a group of experiments which define the function of two sequence elements in the apolipoprotein CIII gene promoter: C3P and N1. The C3P element is a positive regulatory transcriptional element which binds a liver enriched nuclear factor AF-1. The N1 element binds a hepatic factor whose identity and function are unknown. I used electrophoretic gel mobility shift assays, transient transfection assays, in vitro transcription experiments, and in vivo mouse studies to examine the function of these two sequence elements. I found that AF-1 can bind to the C3P element and activate the transcription of the apo CIII promoter; I also defined the nucleotides responsible for AF-1 binding and transcriptional activation in hepa...
AP-2γ is a member of the AP-2 transcription factor family, is highly enriched in the trophoblast cel...
The precise regulation of protein-coding genes, by processes such as transcription, is essential for...
We have previously proposed a molecular interaction between the liver factors that bind to the cycli...
Apolipoprotein CIII (apoCIII), a lipid-binding protein involved in the transport of triglycerides an...
Previously, we showed that when a DNA fragment extending from-3067 to-2734 of the human apolipoprote...
DNase I footprinting analysis of the proximal apoA-I promoter sequences with rat liver nuclear extra...
Hepatic production of apolipoprotein (apo) B-containing lipoproteins provides a means to transport e...
ABSTRACT: BACKGROUND: Multiplex experimental assays coupled to computational predictions are being i...
Objective—Hypertriglyceridemia and fatty liver are common in patients with type 2 diabetes, but the ...
The transcription of acetyl-CoA carboxylase (ACC) gene is driven by two promoters, designated as PI ...
The orphan nuclear receptor hepatocyte nuclear factor 4 (HNF-4) is required for development and main...
Ethanolamine kinase (EK) is the first enzyme in the Kennedy pathway for the biosynthesis of phosphat...
Hepatocyte nuclear factor-1a (HNF-1α) is a transcription factor that plays an important role in regu...
My first project was to investigate the role of Hepatocyte Nuclear Factor 1 (HNF1) and Nuclear Facto...
Thesis (Ph. D.)--University of Rochester. School of Medicine and Dentistry. Dept. of Biochemistry & ...
AP-2γ is a member of the AP-2 transcription factor family, is highly enriched in the trophoblast cel...
The precise regulation of protein-coding genes, by processes such as transcription, is essential for...
We have previously proposed a molecular interaction between the liver factors that bind to the cycli...
Apolipoprotein CIII (apoCIII), a lipid-binding protein involved in the transport of triglycerides an...
Previously, we showed that when a DNA fragment extending from-3067 to-2734 of the human apolipoprote...
DNase I footprinting analysis of the proximal apoA-I promoter sequences with rat liver nuclear extra...
Hepatic production of apolipoprotein (apo) B-containing lipoproteins provides a means to transport e...
ABSTRACT: BACKGROUND: Multiplex experimental assays coupled to computational predictions are being i...
Objective—Hypertriglyceridemia and fatty liver are common in patients with type 2 diabetes, but the ...
The transcription of acetyl-CoA carboxylase (ACC) gene is driven by two promoters, designated as PI ...
The orphan nuclear receptor hepatocyte nuclear factor 4 (HNF-4) is required for development and main...
Ethanolamine kinase (EK) is the first enzyme in the Kennedy pathway for the biosynthesis of phosphat...
Hepatocyte nuclear factor-1a (HNF-1α) is a transcription factor that plays an important role in regu...
My first project was to investigate the role of Hepatocyte Nuclear Factor 1 (HNF1) and Nuclear Facto...
Thesis (Ph. D.)--University of Rochester. School of Medicine and Dentistry. Dept. of Biochemistry & ...
AP-2γ is a member of the AP-2 transcription factor family, is highly enriched in the trophoblast cel...
The precise regulation of protein-coding genes, by processes such as transcription, is essential for...
We have previously proposed a molecular interaction between the liver factors that bind to the cycli...