Photoactive biological systems modify the optical properties of their chromophores, known as spectral tuning. Determining the molecular origin of spectral tuning is instrumental for understanding the function and developing applications of these biomolecules. Spectral tuning in flavin-binding fluorescent proteins (FbFPs), an emerging class of fluorescent reporters, is limited by their dependency on protein-bound flavins, whose structure and hence electronic properties cannot be altered by mutation. A blue-shifted variant of the plant-derived improved light, oxygen, voltage FbFP has been created by introducing a lysine within the flavin-binding pocket, but the molecular basis of this shift remains unconfirmed. We here structurally characteri...
Blue light sensing using flavin (BLUF) protein photoreceptor domains change their hydrogen bond netw...
Photoactivated adenylyl cyclases are powerful tools for optogenetics and for investigating signal tr...
Abstract Background In the 15 years that have passed since the cloning of Aequorea victoria green fl...
iLOV is an engineered flavin-binding fluorescent protein (FbFP) with applications for in vivo cellul...
Flavin is the cofactor for the large and diverse family of proteins called flavoproteins. Upon absor...
Flavin-based fluorescent proteins are a class of fluorescent reporters derived from light, oxygen, a...
Light, oxygen, voltage (LOV) based fluorescent proteins (FPs) represent a promising alternative to f...
The photochemistry and photophysics of organic chromophores in photoresponsive proteins can be tuned...
Light activated proteins are at the heart of photobiology and optogenetics, so there is wide interes...
The proposed mechanisms of photoinduced reactions in the blue light using flavin chromophore photore...
Background: iLOV is a fluorescent flavoprotein engineered from the plant blue light receptor phototr...
Flavin-binding light, oxygen, and voltage (LOV) domains are UVA/blue-light-sensing protein units th...
Photoinduced electron transfer in biological systems, especially in proteins, is a highly intriguing...
Light-Oxygen-Voltage (LOV) domains are conserved parts of photoreceptors in plants, bacteria and fun...
Photoactivated adenylyl cyclases are powerful tools for optogenetics and for investigating signal tr...
Blue light sensing using flavin (BLUF) protein photoreceptor domains change their hydrogen bond netw...
Photoactivated adenylyl cyclases are powerful tools for optogenetics and for investigating signal tr...
Abstract Background In the 15 years that have passed since the cloning of Aequorea victoria green fl...
iLOV is an engineered flavin-binding fluorescent protein (FbFP) with applications for in vivo cellul...
Flavin is the cofactor for the large and diverse family of proteins called flavoproteins. Upon absor...
Flavin-based fluorescent proteins are a class of fluorescent reporters derived from light, oxygen, a...
Light, oxygen, voltage (LOV) based fluorescent proteins (FPs) represent a promising alternative to f...
The photochemistry and photophysics of organic chromophores in photoresponsive proteins can be tuned...
Light activated proteins are at the heart of photobiology and optogenetics, so there is wide interes...
The proposed mechanisms of photoinduced reactions in the blue light using flavin chromophore photore...
Background: iLOV is a fluorescent flavoprotein engineered from the plant blue light receptor phototr...
Flavin-binding light, oxygen, and voltage (LOV) domains are UVA/blue-light-sensing protein units th...
Photoinduced electron transfer in biological systems, especially in proteins, is a highly intriguing...
Light-Oxygen-Voltage (LOV) domains are conserved parts of photoreceptors in plants, bacteria and fun...
Photoactivated adenylyl cyclases are powerful tools for optogenetics and for investigating signal tr...
Blue light sensing using flavin (BLUF) protein photoreceptor domains change their hydrogen bond netw...
Photoactivated adenylyl cyclases are powerful tools for optogenetics and for investigating signal tr...
Abstract Background In the 15 years that have passed since the cloning of Aequorea victoria green fl...