An LTR-based Quantitative PCR (qPCR) assay was modified and optimized for the quantification of total HIV-1 nucleic acids in plasma and PBMC. TaqMan qPCR primers and probes were designed against the NCBI/LANL HIV-1 compendium database by analyzing sequences used in assays for sensitive cross-clade detection of HIV-1 as reported in the literature and elucidating regions of improved cross-subtype specificity. Inosine and mixed nucleotide bases were included at polymorphic sites. Real-time RT-qPCR and qPCR were performed on plasma viral RNA and cellular lysates. A step-up amplification approach to allow binding of primers across polymorphic regions showed improved sensitivity compared to universal cycling. Unlike a lead competing laboratory-de...
An ultrasensitive version of an 'in-house' reverse transcription-competitive polymerase chain reacti...
Although antiretroviral treatment availability has improved, the virological monitoring of patients ...
BACKGROUND: The persistence of proviral human immunodeficiency virus type 1 (HIV-1) DNA reservoir re...
AbstractSignificant advances have been made in the molecular assays used for the detection of human ...
The correct diagnosis andmonitoring of HIV-1 group O (HIV-O) infection are essential for appropriate...
The CDC estimates that there are currently over 1 million people living with human immunodeficiency ...
Introduction: The latent reservoir is the main barrier on the road to HIV cure, and clinical approac...
Quantification of the viral burden and identification of drug resistant mutations are important labo...
Quantification of human immunodeficiency virus type-1 (HIV-1) proviral DNA is increasingly used to m...
Background Most of the quantitation assays for HIV-1 RNA used currently are designed and optimized f...
Quantification of Human Immunodeficiency Virus (HIV-1) RNA plays a vital role in diagnosing and moni...
The commercial assays commonly used to quantify plasma human immunodeficiency virus type 1 (HIV-1) R...
Although antiretroviral treatment availability has improved, the virological monitoring of patients ...
A method for quantitating human immunodeficiency virus type 1 plasma viremia may be useful in monito...
Background: The quantitative measurement of various HIV-1 DNA forms including total, unintegrated an...
An ultrasensitive version of an 'in-house' reverse transcription-competitive polymerase chain reacti...
Although antiretroviral treatment availability has improved, the virological monitoring of patients ...
BACKGROUND: The persistence of proviral human immunodeficiency virus type 1 (HIV-1) DNA reservoir re...
AbstractSignificant advances have been made in the molecular assays used for the detection of human ...
The correct diagnosis andmonitoring of HIV-1 group O (HIV-O) infection are essential for appropriate...
The CDC estimates that there are currently over 1 million people living with human immunodeficiency ...
Introduction: The latent reservoir is the main barrier on the road to HIV cure, and clinical approac...
Quantification of the viral burden and identification of drug resistant mutations are important labo...
Quantification of human immunodeficiency virus type-1 (HIV-1) proviral DNA is increasingly used to m...
Background Most of the quantitation assays for HIV-1 RNA used currently are designed and optimized f...
Quantification of Human Immunodeficiency Virus (HIV-1) RNA plays a vital role in diagnosing and moni...
The commercial assays commonly used to quantify plasma human immunodeficiency virus type 1 (HIV-1) R...
Although antiretroviral treatment availability has improved, the virological monitoring of patients ...
A method for quantitating human immunodeficiency virus type 1 plasma viremia may be useful in monito...
Background: The quantitative measurement of various HIV-1 DNA forms including total, unintegrated an...
An ultrasensitive version of an 'in-house' reverse transcription-competitive polymerase chain reacti...
Although antiretroviral treatment availability has improved, the virological monitoring of patients ...
BACKGROUND: The persistence of proviral human immunodeficiency virus type 1 (HIV-1) DNA reservoir re...