The Eocene–Oligocene Transition (EOT) marks the onset of the Antarctic glaciation and the switch from greenhouse to icehouse climates. However, the driving mechanisms and the precise timing of the EOT remain controversial mostly due to the lack of well-dated stratigraphic records, especially in continental environments. Here we present a cyclo-magnetostratigraphic and sedimentological study of a ∼ 7.6 Myr long lacustrine record spanning the late Eocene to the earliest Oligocene, from a drill core in the Rennes Basin (France). Cyclostratigraphic analysis of natural gamma radiation (NGR) log data yields duration estimates of Chrons C12r through C16n.1n, providing additional constraints on the Eocene timescale. Correlations between the orbital...