An intrinsically disordered nascent protein interacts with specific regions of the ribosomal surface near the exit tunnel

Guzman-Luna et al. present a crosslinking analysis of the interaction of unstructured nascent chains with ribosomal proteins near the nascent polypeptide exit tunnel of the ribosome. They further analyze the dependence of these interactions on the peptide length, surface charge and ionic strength (including Mg+2 concentration), facilitating the understanding of co-translational protein folding and misfolding/aggregation