Formación de multicapas poliméricas para la constitución de implantes destinados a la liberación de compuestos biológicos que estimulen la respuesta celular

The objective of this work is the development of multilayer polymeric systems based on biocompatible polymers using the layer-by-layer technique and the inclusion of proteins in these systems. This development is proposed for application in the biomedical field as biomaterials that function as implants. The inclusion of proteins in the materials that make up the implants and their subsequent controlled release is intended to achieve a localized response of the organism. In order to carry out these polymeric systems, the LbL technique is used by immersion in solutions of polymers with opposite charge. The BMP-2 protein is chosen for the binding and release study and its inclusion in the polymeric system is carried out with the same LbL procedure. On the one hand, the behavior of the polymers composing the system is analyzed with a quartz crystal microbalance. On the other hand, the correct binding of the protein embedded in the polymeric system is analyzed by means of release and detection assays by ELISA technique. After the analysis of the different assays, the results have confirmed the effectiveness of the layer by layer technique for the formation of polymeric multilayers capable of incorporating and subsequently releasing biological compounds. The protein release results collected for the first eight days of incubation indicate a more controlled release of BMP-2 protein over time in these implants formed by polymeric multilayers