Functional Variables of Bull Sperm Associated with Cryotolerance

Alicia GILMORE
Mustafa HITIT
Muhammet Rasit UGUR
Thu Tran Nhat DINH
Wei TAN
Dean JOUSAN
Molly NICODEMUS
Einko TOPPER
Abdullah KAYA
Erdogan MEMILI

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Publication date

May 2021

DOI

10.9775/kvfd.2021.25653

Publisher

Kafkas University

Journal

Kafkas Universitesi Veteriner Fakultesi Dergisi

Abstract

The objective of this study was to ascertain sperm population and cellular characteristics as well as total antioxidant capacity in spermatozoa from Holstein bulls with Good (11 bulls) and Poor (5 bulls) cryotolerance. Post-thaw sperm kinetics were evaluated using CASA, membrane integrity was assessed via HOS test, and DNA fragmentation was measured using the HaloSperm kit. Data were analyzed using principal component analysis. The spermatozoa from Good bulls had a higher number of cells with intact membranes (P=0.029), non-fragmented DNA (P=0.018), and post-thaw viability (P<0.001) compared to sperm cells from Poor cryotolerance bulls. Sperm cells from Good bulls also had a faster average path velocity (P=0.017) and straight-line velocity ...