Lignocellulosic Corn Stover Biomass Pre-Treatment by Deep Eutectic Solvents (DES) for Biomethane Production Process by Bioresource Anaerobic Digestion

The valorization study of the largely available corn stover waste biomass after pretreatment with deep eutectic solvent (DES) for biomethane production in one-liter glass bioreactors by anaerobic digestion for 21 days was presented. Ammonium thiocyanate and urea deep eutectic solvent pretreatments under different conditions in terms of the components ratio and temperature were examined on corn stover waste biomass. The lignocellulose biomass was characterized in detail for its chemistry and morphology to determine the effect of the pretreatment on the natural biocomposite. Furthermore, the implications on biomethane production through anaerobic digestion with different loadings of corn stover biomass at 35 g/L and 50 g/L were tested. The results showed an increase of 48% for a cumulative biomethane production for a DES-pretreated biomass, using a solid-to-liquid ratio of 1:2 at 100 °C for 60 min, which is a strong indication that DES-pretreatment significantly enhanced biomethane production