This paper is dedicated to the experimental validation of a cascade control strategy for simultaneously regulating the glucose and biomass levels in continuously perfused HEK-293 cell cultures. The inner loop consists of a partial feedback linearization, which requires the estimation of the biomass specific growth rate and glucose uptake rate. This latter task is achieved by sliding mode observers, which do not require a priori process knowledge in the form of a process model. The linearized process is then regulated by the outer loop, including two classical PI controllers with autotuning. The four manipulated variables are a feed flow rate with low glucose concentration, another feed flow with a higher glucose content, a bleed flow, and a...
This paper describes the development of improved control strategies for the standard environmental c...
This paper describes the development of improved control strategies for the standard environmental c...
The objective of this work was to evaluate the performance of a feedback glucose control strategy (t...
This paper presents a multivariable robust control structure for perfusion animal cell cultures. The...
The progressive acceleration of continuous bioprocessing method has become the focal point of advanc...
This paper presents the development of a multivariable nonlinear adaptive controller for perfusion b...
This paper addresses the control of biomass growth rate in fed-batch bioreactors. The main difficult...
A main problem in controlling bioprocesses is the lack of manipulated variables. Batch and fed-batch...
The controlled-fed perfusion culture, integrating the advantages of both the fed-batch and perfusion...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
This paper describes the development of improved control strategies for the standard environmental c...
This paper describes the development of improved control strategies for the standard environmental c...
This paper describes the development of improved control strategies for the standard environmental c...
The objective of this work was to evaluate the performance of a feedback glucose control strategy (t...
This paper presents a multivariable robust control structure for perfusion animal cell cultures. The...
The progressive acceleration of continuous bioprocessing method has become the focal point of advanc...
This paper presents the development of a multivariable nonlinear adaptive controller for perfusion b...
This paper addresses the control of biomass growth rate in fed-batch bioreactors. The main difficult...
A main problem in controlling bioprocesses is the lack of manipulated variables. Batch and fed-batch...
The controlled-fed perfusion culture, integrating the advantages of both the fed-batch and perfusion...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
Accurate control of the specific growth rate (µ) of microorganisms is dependent on the ability to qu...
This paper describes the development of improved control strategies for the standard environmental c...
This paper describes the development of improved control strategies for the standard environmental c...
This paper describes the development of improved control strategies for the standard environmental c...
The objective of this work was to evaluate the performance of a feedback glucose control strategy (t...