Sintesis Dan Uji Aktivitas Antibakteri Senyawa Kalkon (E)-3-(4-(Dimethylamino)Phenyl)-1-Phenylprop-2-En-1-One Terhadap Bakteri Kontaminan Produk Darah

Chalcone compounds and their derivatives are known to have various pharmacological activities, one of which is antibacterial activity. This activity is strongly influenced by the type of bound substituent and the location of the substituent on the structure of the chalcone compound. The purpose of this study was to synthesize chalcone compounds with the characteristics of the dimethylamino substituent on ring B at the para ((E)-3-(4-(dimethylamino)phenyl)-1-phenylprop-2-en-1-one) position and to test the activity of an antibacterial compound of chalcone against bacterial isolates of blood product contaminants. Antibacterial activity testing was focused on bacterial isolates derived from blood products, because the use of blood product bacterial isolates for antibacterial testing was still not widely used, generally the test bacteria used were ATCC bacterial isolates. The antibacterial activity test method used was the paper disc diffusion method, the antibacterial activity of chalcone compounds was known through the formation of a clear zone around the disc paper called the inhibition zone for bacterial growth. The chalcone compound (E)-3-(4-(dimethylamino)phenyl)-1-phenylprop-2-en-1-one was successfully synthesized through a condensation reaction, which obtained a yellow crystalline solid with a yield of 81.67%. The results of the activity test of chalcone compounds on several blood product contaminants showed that this compound had good enough activity in inhibiting the growth of Staphylococcus aureus bacteria with an inhibition zone of 8.9 mm at a concentration of 25 ppm.Senyawa kalkon dan turunannya dikenal memiliki berbagai aktivitas farmakologis, salah satunya adalah aktivitas antibakteri. Aktivitas ini, sangat dipengaruhi oleh jenis subtituen yang terikat serta letak subtituen tersebut pada struktur senyawa kalkon. Tujuan penelitian ini adalah melakukan sintesis senyawa kalkon dengan karakteristik subtituen dimetilamino pada ring B di posisi para ((E)-3-(4-(dimethylamino)phenyl)-1-phenylprop-2-en-1-one) serta melakukan uji aktivitas antibakteri senyawa kalkon terhadap isolat bakteri kontaminan produk darah. Pengujian aktivitas antibakteri difokuskan terhadap isolat bakteri yang berasal dari produk darah, dikarenakan penggunaan isolat bakteri produk darah untuk pengujian antibakteri masih belum banyak digunakan, umumnya bakteri uji yang digunakan adalah isolat bakteri ATCC. Metode pengujian aktivitas antibakteri yang digunakan adalah metode difusi kertas cakram, aktivitas antibakteri senyawa kalkon diketahui melalui terbentuknya zona bening di sekitar kertas cakram yang disebut zona hambat pertumbuhan bakteri. Senyawa kalkon (E)-3-(4-(dimethylamino)phenyl)-1-phenylprop-2-en-1-one berhasil disintesis melalui reaksi kondensasi, dimana diperoleh padatan kristal berwarna kuning dengan rendemen sebesar 81,67%. Hasil uji aktivitas senyawa kalkon pada beberapa bakteri kontaminan produk darah menunjukkan bahwa senyawa ini memiliki aktivitas cukup baik dalam menghambat pertumbuhan bakteri Staphylococcus aureus dengan zona hambat mencapai 8,9 mm pada konsentrasi 25 ppm