Synthesis of Cibacron Blue F3GA-coupled magnetic PMMA nanospheres and their use for protein affinity separation

BACKGROUND: New magnetic carrier separation technologies, capable of treating dilute solutions in large-scale processes, even in the presence of biological debris, are necessary for the future development of biotechnology. Non-porous magnetic carriers are more resistant to fouling, show better mass transfer and have lower non-specific adsorption than porous carriers. Nanosized magnetic carriers have a surface area comparable to that of typical macroporous resins, and therefore their application has advantages